FIG. 2.

Replication of recombinant viruses in tissue culture. Subconfluent RS cell monolayers were infected with 1 PFU/cell of vP35, vP40, vIL-4, dbl-p, or KOS as described in Materials and Methods. Total virus was harvested at the indicated times postinfection by two cycles of freeze-thawing. The amount of virus at each time point for each virus was determined by standard plaque assay on RS cells. Each point represents mean ± the SEM from three experiments.