TABLE 4.

Source of cytokine production by splenocytes from mice vaccinated with recombinant viruses expressing different cytokine genes^a

Vaccine	Amt (%) of:
	IL-2 produced by:		IFN-γ produced by:
	CD4+ T cells	CD8+ T cells	CD4+ T cells	CD8+ T cells
vP35	21	79	42	58
vP40	35	65	49	51
vP35+vP40	32	68	50	50
vIL-4	51	49	49	51
dbl-p	47	53	53	47
KOS	52	48	51	49

^aMice were immunized with each recombinant virus, and spleens were harvested 3 weeks after the third immunization. Single cell suspensions of CD4⁺, CD8⁺, or pooled CD4⁺ and CD8⁺ T cells were prepared and subjected to in vitro stimulation for 72 h with 5 PFU of UV-inactivated HSV-1 McKrae/cell. The presence of cytokines in the supernatant was determined by ELISA. Each number represents the percentage of each cytokine that was produced by CD4⁺ T cells or CD8⁺ T cells in relation to the amount produced by both T cells from three experiments.