FIG. 3.

Effects of stem a mutations on viral RNA stability. HeLa S10 translation-replication reaction mixtures (150 μl) containing equimolar amounts of helper RNA and the indicated ³²P-labeled viral RNA were incubated for 4 h at 34°C, and 20-μl samples of the reaction mixture were removed at the indicated time points. The ³²P-labeled viral RNA remaining at each time point was determined by gel electrophoresis (A, C, and E) or by precipitation in trichloroacetic acid (B, D, and F) as described in Materials and Methods.