FIG. 6.

Effect of altering the 5′-terminal sequence on negative-strand RNA synthesis. PIRCs isolated from HeLa S10 reactions containing helper RNA and the indicated template RNA were resuspended in reaction mixtures containing [³²P]CTP and incubated at 37°C for 8 min. The ³²P-labeled product RNAs synthesized in these reactions were characterized by gel electrophoresis and quantitated as described for Fig. 5.