TABLE 1.

Effects of stem a mutations on virus production in HeLa S10 translation-replication reactions

RNA transcript	PFU/ml	Plaque diam (mm)a
Rz-PV1	1.3 × 106	2.1 ± 0.4
Rz-PV1mut2	NDb	ND
Rz-PV1mut7	2.5 × 101	1.0 ± 0.5
Rz-PV1	5.0 × 105	2.3 ± 0.4
Rz-PV1mut10	5.0 × 104	1.9 ± 0.3
Rz-PV1	1.3 × 106	2.1 ± 0.4
Rz-PV1mut3	9.6 × 104	2.0 ± 0.3
Rz-PV1mut4	4.3 × 104	2.1 ± 0.4
Rz-PV1mut8	8.8 × 102	0.6 ± 0.2

^aPlaque diameter was calculated as an average of at least 15 plaques, except Rz-PV1mut7 RNA, which produced only 5 plaques.

^bND, no detectable plaques were obtained.