Figure 2.

CPEB2 induces G1 cell cycle arrest. (A,B) Cell cycle distribution in U87 cells with CPEB2 stable overexpression or not. (C,D) Cell cycle distribution in U87 cells with CPEB2 stable knockdown or not. Indicated cells were starved in medium containing no serum for 48 h, and then released into complete growth medium for 24 h. After release, harvested the cells and analyzed by flow cytometry. The dataset of (A,C) is representative example of triplicate experiments. Column graph of (B,D) was mean ± SD of three independent experiments. All values are mean ± SEM. Student t-tests were used. ***P < 0.001. SEM, standard error of the mean.