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. 2023 Dec 29;13:23103. doi: 10.1038/s41598-023-50848-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CPEB2 upregulates p21 expression by increasing its mRNA stability. (A,B) The protein expression of p21 in CPEB2 overexpression or knockdown U87 cells were detected by western blot. (C) The localization of CPEB2 in CPEB2 overexpression U87 cells was detected by indirect immunofluorescence assay. (D) The nuclei and cytoplasm of U87 cells were isolated, and CPEB2 protein expression levels were detected by western blot. (E,G) RT-qPCR of p21 mRNA expression in CPEB2 overexpression or knockdown U87 cells. (F,H) The levels of p21 transcript was measured by RT-qPCR in CPEB2 overexpression or knockdown U87 cells treated with 10 μg/mL actinomycin D for the indicated times. (I) CPEB2 interaction with p21 transcript in vitro. The lysates of CPEB2 overexpression U87 cells were immunoprecipitated with CPEB2 antibodies or control IgG, and RT-qPCR was used to measure the transcript levels of p21 and GAPDH precipitated by CPEB2 or IgG immunocomplexes. All values are mean ± SEM. Two way ANOVA followed by Tukey's multiple comparison test or Student t-tests were used. *P < 0.05, **P < 0.01, and ***P < 0.001. SEM standard error of the mean.