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. 2023 Dec 29;13:23103. doi: 10.1038/s41598-023-50848-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The anti-tumor effect of CPEB2 is partially dependent on upregulating p21 in glioma. (A) p21 overexpression plasmids were transfected into CPEB2 knockdown U87 cells. CPEB2, p21 and Actin protein expression were detected by western blot. (B) p21 overexpression abrogated the pro-proliferative effect of CPEB2 knockdown in CCK-8 assay. (C) p21 siRNA were transfected into CPEB2 overexpression U87 cells. CPEB2, p21 and Actin protein expression were detected by western blot. (D) silencing of p21 reversed glioma cell proliferation inhibition of CPEB2 overexpression in CCK-8 assay. (E,F) Indicated cells were stained with EDU and DAPI. The red color indicates EDU-positive nuclei. Column graph was mean ± SEM of three independent experiments. (G) p21 overexpression abrogated the G1/S phase transition effect of CPEB2 knockdown in flow cytometry assay. (H) silencing of p21 reversed the G1 cell cycle arrest effect of CPEB2 overexpression in flow cytometry assay. All values are mean ± SEM. Two way ANOVA followed by Tukey's multiple comparison test or Student t-tests were used. **P < 0.01, ***P < 0.001 vs vector or shNC; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs shCPEB2; ^&&P < 0.01, ^&&&P < 0.001 versus CPEB2 + siNC. SEM standard error of the mean.