FIG. 10.

Northern blot of infections with sat-RNA mutant transcripts. Lane 1, total RNAs extracted from C. amaranticolor inoculated with in vitro transcripts of BBSV RNA plus full-length sat-RNA from pMBS-M4 (see Fig. 7); lane 2, Mut-3 sat-RNA transcripts; lane 3, Mut-2 sat-RNAs; lane 4, pUBS-Md3 sat-RNAs; lane 5, Mut-1 sat-RNA transcripts; lane 6, extracts from leaves inoculated with an in vitro transcript of BBSV-N genomic RNA alone. The diagrams at the right represent each mutated sat-RNA used for inoculation. M, mixture of monomeric, dimeric, trimeric, and tetrameric sat-RNAs used as markers. The numbers on the diagrams represent the terminal nucleotides of the transcript or the number of extra nucleotides at the 3′ end. Note that the rRNA loading lanes reveal that the total RNAs in lane 2, 3, 4, and 5 were twice as concentrated as those in lanes 1 and 6.