FIG. 3.

cDNA synthesis and sequencing of BBSV satellite RNA. (A) Sequencing strategy using cDNA plasmids (pGBS-P12, pGBS-P40, and pGBS-P20) that were amplified with oligo(dT)₁₅ primers and plasmids (pGBS-R2, pGBS-R27, and pGBS-R19) that were recovered after priming with random primers. (B) Complete nucleotide sequence of the 615-nt BBSV sat-RNA. The 12 underlined bases at the 5′ end were determined by RACE RT-RCR as shown in Fig. 5A.