TABLE 1.
List of oligonucleotides used for PCR
| Oligonucleotide | Sequence (5′-3′) | Structure and/or positiona |
|---|---|---|
| BB-S1 | CCTCACCTTCCCTGGCAGAC | nt 303 to 284 |
| BB-S2 | CCCGGGTAGCAATTAATTGC | CCC, nt 615 to 599 |
| BB-S3 | TAATACGACTCACTATAGGAAAGCTACGTGGTCATGGTAG | T7 promoter, G, nt 1 to 22 |
| BB-S4 | GAAAGCTACGTGGTCATGGTAG | nt 1 to 22 |
| BB-S5 | GTTCACATGATAACCGTTGG | nt 460 to 479 |
| BB-S7 | TAATACGACTCACTATAGGGGTAGCAATTAATTGC | T7 promoter, G, nt 615 to 599 |
| BB-S10 | GATATCAAATACTACCTCCC | EcoRV, nt 277 to 258 |
| BB-S11 | TAATACGACTCACTATAGCTACGTGGTCATGGTAGAG | T7 promoter, G, nt 6 to 24 |
| BB-S12 | GCAATTAATTGCTTTATAGC | nt 610 to 591 |
| BB-S13 | CTACGTGGTCATGGTAGAG | nt 6 to 24 |
| BB-31 | GCTCT(A/C)GAGAATTCCC(G)15 | Oligo(dG) anchor |
| MD18-T1 | ATCGTCGACCTGCAGGCATG | nt 425 to 406 in plasmid pMD18-T |
a
Numbers correspond to the sat-RNA nucleotide positions, and a reverse order of numbers indicates that the primer is complementary to the sat-RNA.