TABLE 3.
Genes or genomic regions recurrently hit by rAAV2 integration
| Target gene or genomic region
|
No. of integration eventsa
|
||||||
|---|---|---|---|---|---|---|---|
| Symbol | Name | Size (kb) | Mouse
|
Total (no. in RefSeq gene)b | |||
| A | B | C | D | ||||
| Bhmt | C betaine-homocysteine methyltransferase | 20.7 | 1 | 1 | 2 (2) | ||
| Cmas | Cytidine monophospho-N-acetylneuraminic acid synthetase | 28.7 | 1 | 1 | 2 (2) | ||
| Gsk3b | Glycogen synthase kinase 3 beta | 198.1 | 2 | 2 (2) | |||
| Gtf2i | General transcription factor III | 76.9 | 1 | 1 | 2 (2) | ||
| LOC381596c | LOC381596 | 13.8 | 2 | 2 | |||
| Myo6 | Myosin IV | 91.9 | 1 | 1 | 2 (2) | ||
| Nedd4I | Neural precursor cell expressed, developmentally down-regulated gene 4 like | 28.1 | 1 | 1 | 2 (2) | ||
| Sdccag33c | Serologically defined colon cancer antigen 33 | 2.0 | 1 | 2d | 3 | ||
| Spag9 | Sperm-associated antigen 9 | 127.3 | 1 | 1 | 2 (2) | ||
| Ubc | Ubiquitin C | 1.5-26.9 | 2 | 1 | 3 (3) | ||
| Ubtf | Upstream binding transcription factor; RNA polymerase I | 10.5 | 2e | 1 | 3 (2) | ||
| Usp10 | Ubiquitin specific protease 10 | 46.7 | 2 | 2 (2) | |||
| 1300002F13Rik | Riken cDNA 1300002F13 gene | 13.8 | 2 | 1 | 3 (3) | ||
| 45S pre-rRNA gene repeatc | 45.3 × 200f | 2 | 2 | 3 | 3 | 10 | |
a
Integrations into genes (with no footnote) and into upstream regulatory regions (indicated by footnote d or e) are listed.
b
The numbers in parentheses represent integrations in RefSeq genes.
c
These are not RefSeq genes. LOC381596 and Sdccag33 are identified as genes in the NCBI Map Viewer. The Sdccag33 gene is classified as a known gene according to the definition by UCSC Genome Bioinformatics.
d
One of the two integrations found in mouse C landed 1.4 kb upstream of the Sdccag33 gene, as defined by NCBI.
e
One of the two integrations found in mouse B landed 0.3 kb upstream of the Ubtf gene.
f
There are approximately 200 copies of the 45S pre-rRNA repeats per haploid mouse genome.