TABLE 4.
Host chromosomal changes associated with rAAV2 integration
| Target site deletion
|
Target site duplication
|
Translocation
|
|||
|---|---|---|---|---|---|
| Size of deletion (bp) | No. of events | Size of duplication (bp) | No. of events | Chromosome | No. of events |
| 0-10 | 11 | 1 | 1 | t(3:12) | 1 |
| 11-30 | 27 | 2 | 2 | t(3:14) | 1 |
| 31-100 | 40 | 3 | 3 | t(6:15) | 1 |
| 101-300 | 51 | 4 | 1 | t(9:17) | 1 |
| 301-1,000 | 34 | 12-13 | 2 | t(11:19) | 1 |
| 1,001-3,000 | 27 | 40-79 | 3 | ||
| >3,000 | 10 | 277 | 1 | ||
| Total | 200/218 (91.7%)a | 13/218 (6%) | 5/218 (2%) | ||
a
Vector-flanking mouse genomic sequences were determined in both sides and unambiguously mapped in 218 integration events. The frequencies of deletions, duplications, and translocations are calculated based on this number. Median target site deletion size, 205 bp; maximum, 5.5 Mb.