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. 2023 Dec 29;7(3):e202302341. doi: 10.26508/lsa.202302341

Figure S5. CXCL8 and IL6 mRNA abundance is not significantly modified in smokers and COPD AE, whereas increased epithelial release of inflammatory cytokines partly persists in COPD AE.

Figure S5.

(A, B) Left panel: CXCL8 gene expression was enhanced in smokers and mild-to-moderate COPD in early and short-term ALI cultures, respectively, but no striking difference was observed in later time periods or in (very) severe COPD. Right panel: no difference was observed in IL6 gene expression in smokers and COPD-derived ALI-AE as compared with non-smokers. (C) IL-8/CXCL-8 production by the reconstituted ALI-AE is increased in early, short-term, and mid-term ALI-AE from COPD patients, but disappears afterwards, although a non-significant upward trend persists. (D) IL-6 production by the reconstituted ALI-AE is increased in Smo and COPD and persists in long-term cultures. * indicates a P-value of less than 0.05, whereas **** indicates P-values of less than 0.0001. Bars indicate mean ± SD (A [left], B) and median ± interquartile range (A [right], C). AE, airway epithelium; ALI, air-liquid interface; COPD, chronic obstructive pulmonary disease; IL, interleukin; NS, non-smokers; ns, not significant; SD, standard deviation; SEM, standard error of the mean; Smo, smokers; w, weeks.