Subject	Biological sciences
Specific subject area	Structural biology
Data format	Raw, Analyzed
Type of data	X-ray diffraction data, Table, Image, Graph, Figure
Data collection	Synchrotron: Pohang Light Source II (PLS-II) Beamline: 1C X-ray wavelength: 15.48 keV X-ray bandwidth (ΔE/E): 1.2 % Photon flux: ∼5 × 1012 photons/second Beam size: 70 × 80 μm (full width at half maximum, vertical x horizontal) Sample delivery: viscous medium-based injection via syringe and syringe pump Sample flow rate: 1 μL/min Detector: Pilatus3S 2 M (DECTRIS). Data acquisition: 10 or 20 Hz Data collection temperature: 26 ± 0.5°
Data source location	Institution: Kookmin University City/Town/Region: Seoul Country: Republic of Korea
Data accessibility	1. Raw data diffraction images Repository name: ZENODO 1) Data 1 (LysEdge50): X-ray exposed to the edge of the injection stream with an X-ray exposure time of 50 ms - Digital Object Identifier: https://doi.org/10.5281/zenodo.7240990 - Direct URL to data: https://zenodo.org/records/7240990 2) Data 2 (LysCenter50): X-ray exposed to the center of the injection stream with an X-ray exposure time of 50 ms. - Digital Object Identifier: https://doi.org/10.5281/zenodo.7239100 - Direct URL to data: https://zenodo.org/records/7239100 3) Data 3 (LysEdge100): X-ray exposed to the edge of the injection stream with an X-ray exposure time of 100 ms - Digital Object Identifier: https://doi.org/10.5281/zenodo.7242977 - Direct URL to data: https://zenodo.org/records/7242977 4) Data 4 (LysCenter100): X-ray exposed to the center of the injection stream with an X-ray exposure time of 100 ms. - Data identification number: https://doi.org/10.5281/zenodo.7242973 - Direct URL to data: https://zenodo.org/records/7242973 2. Structure factor and coordinate Repository name: Protein Data Bank 1) Data 1: X X-ray exposed to the edge of the injection stream with an X-ray exposure time of 50 ms - PDB code: 8H8T - Data identification number: https://doi.org/10.2210/pdb8H8T/pdb - Direct URL to data: https://www.rcsb.org/structure/8H8T 2) Data 2: X-ray exposed to the center of the injection stream with an X-ray exposure time of 50 ms. - PDB code: 8H8U - Data identification number: https://doi.org/10.2210/pdb8H8U/pdb - Direct URL to data: https://www.rcsb.org/structure/8H8U
	3) Data 3: X-ray exposed to the edge of the injection stream with an X-ray exposure time of 100 ms - PDB code: 8H8V - Data identification number: https://doi.org/10.2210/pdb8H8V/pdb - Direct URL to data: https://www.rcsb.org/structure/8H8V 4) Data 4: X-ray exposed to the center of the injection stream with an X-ray exposure time of 100 ms. - PDB code: 8H8W - Data identification number: https://doi.org/10.2210/pdb8H8W/pdb - Direct URL to data: https://www.rcsb.org/structure/8H8W
Related research article	Y. Kim, K.H. Nam, Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II, Crystals (2022) [1]https://doi.org/10.3390/cryst12111637