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. 2005 Mar;79(6):3231–3242. doi: 10.1128/JVI.79.6.3231-3242.2005

FIG. 1.

FIG. 1.

MDBK cells acutely infected with ncp BVDV are protected from CPE when superinfected with homologous cp BVDV but not with vesicular stomatitis virus. (A) Experimental procedures to examine superinfection exclusion in ncp BVDV-infected MDBK cells. (B and C) Naïve MDBK cells were first mock infected or infected with ncp NADLJiv90− BVDV. Subsequently, the cells were either mock infected or infected with only cp NADL BVDV or cp vesicular stomatitis virus (VSV). At 12 h postinfection, ncp NADLJiv90−-infected cells were washed three times with medium and either mock superinfected or superinfected with cp NADL BVDV or cp vesicular stomatitis virus for 1 h. The + and − above each column of plates indicate infection and mock infection, respectively, with the particular virus shown at the top left. The cells were overlaid with agarose (B and C, plates 1 to 4) or incubated in liquid culture medium (B and C, plates 5 to 8). The cells were fixed and stained with crystal violet to visualize live cells surviving CPE after 5 days (plaque assay) or 3 days (liquid overlay).