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Minimum spanning tree (MST) and host association of sequence types (ST). The ST found in this study were combined with corresponding ST entries from the pubMLST database (pubmlst.org/organisms/leptospira-spp) with information on host in a MST analysis using Bionumerics 8.1. The fox and the three raccoon samples were determined in this study. The new ST375 found in fox is closely related to ST24 as indicated by the branch length and a single allele difference.
