TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or characteristicsa | Source and/or reference |
|---|---|---|
| H. pylori | ||
| HPK5 | Wild type | Gastric ulcer, 17 |
| CPY2052 | Wild type | Gastric ulcer, 17 |
| CPY3401 | Wild type | Gastric ulcer, 17 |
| CPY1113 | Wild type | Duodenal ulcer |
| HPKT510 | HPK5 derivative; xylE-kan in cdrA; Kmr | This study |
| HPKT5L2 | HPK5 derivative; xylE-kan in ureF; Kmr | This study |
| HPKT510R | HPKT510 derivative; cat in ureF, intact cdrA; Cmr | This study |
| E. coli | ||
| NM554 | MC1061 recA13 | Stratagene |
| DH5α | F− φ80dlacZΔM15 Δ(argF-lac)U169 deoR recA1 endA1 hsdR17(rK− mK+) supE44 thi-1 gyrA96 relA1 | GIBCO-BRL |
| XL1-Blue | hsdR17 supE44 recA1 endA1 gyrA46 thi relA1 lac/F′ [proAB+ lacIqlacZΔM15::Tn10(Tetr)] | Stratagene |
| JM105 | endA1 supE sbcB15 thi rpsL Δ(lac-proAB)/F′ [traD36 proAB+ lacIqlacZΔM15] | Pharmacia |
| Plasmids | ||
| pMT5047 | SuperCos1 derivative excluding the 2,160-bp NruI fragment; Apr | This study |
| pHP802 | 8-kb fragment with the urease gene cluster of strain UMAB41; pACYC184 replicon; Apr | 15 |
| pHPT177 | 12.8-kb fragment with the urease gene cluster of HPK5; pBR322 replicon; Apr | This study |
| pS4-1 | 3.5-kb fragment with the cdrA, pBluescript II KS(−) replicon; Apr | This study |
| pMT5047 | xylE-kan (3.13 kb), pBR322 replicon; Apr, Kmr | This study |
| pTA10 | xylE-kan (3.13 kb) in cdrA of pS4-1; Apr, Kmr | This study |
| p177L2 | xylE-kan (3.13 kb) in ureF of pHPT177; Apr, Kmr | This study |
| pTA40 | cat (1.1 kb) in ureF of pHPT177; Apr, Cmr | This study |
| pBSC103 | cat (1.2 kb) on pBluescript; Apr, Cmr | D. E. Berg |
| pBluescript | 3.2-kb phagemid vector; Apr | Stratagene |
| pBluescript II KS(−) | 2.9-kb cloning vector; Apr | Stratagene |
| pBR322 | 4.4-kb cloning vector; ColE1 replicon; Apr, Tcr | Takara |
a
Abbreviations: Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Tcr, tetracycline resistance; Kmr, kanamycin resistance.