Table 2.
Primer sequences used for Q-RT-PCR.
| Gene name | Q-RT-PCR primer |
mRNA/CDS NCBI Accession | |
|---|---|---|---|
| Forward（5′-3'） | Reverse（5′-3'） | ||
| β-actin (Hum) | GCACTCTTCCAGCCTTCCTT | AATGCCAGGGTACATGGTGG | X00351.1 |
| TLR4 (Hum) | GTGTCCCAGCACTTCATCCA | GGGTCTTCTCCACCTTCTGC | U88880.1 |
| MyD88 Hum) | GTCTCCTCCACATCCTCCCT | CAGTTGCCGGATCTCCAAGT | U70451.1 |
| NF-κB (Hum) | TTTCAACCACAGATGGCACT | TGCTGGTCCCACATAGTTGC | EF216688.1 |
| IL-4 (Hum) | GCTTCCCCCTCTGTTCTTCC | CTGCTCTGTGAGGCTGTTCA | M13982.1 |
| IL-13 (Hum) | GCAATGGCAGCATGGTATGG | CTCTGGGTCTTCTCGATGGC | L06801.1 |
| MCP1 (Hum) | GCTCATAGCAGCCACCTTCA | CTTTGGGACACTTGCTGCTG | NM_002982.4 |
| TNF-α (Hum) | CTTCTGCCTGCTGCACTTTG | GTCACTCGGGGTTCGAGAAG | NM_000594.4 |
| TSLP (Hum) | CGGCCACATTGCCTTACTGA | GGCGAACATTTCTTTGGCGA | AY037115.1 |
| β-actin (Rat) | GCTGTGCTATGTTGCCCTAGAC | CCGCTCATTGCCGATAGTGATG | NM_031144.3 |
| TLR4 (Rat) | CCAGAGCCGTTGGTGTATCT | AGAAGATGTGCCTCCCCAGA | NM_019178.1 |
| MyD88 (Rat) | CGGAGGAGATGGGTTTCGAG | CCAGGCATCCAACAAACTGC | NM_198130.1 |
| NF-κB (Rat) | CATACGCTGACCCTAGCCTG | TTTCTTCAATCCGGTGGCGA | AY307375.1 |
| IL-6 (Rat) | CCCAACTTCCAATGCTCTCCT | GGTTTGCCGAGTAGACCTCAT | NM_012589.2 |
| TSLP (Rat) | TTATCCTGCAAGTGGTACGGC | TCACAGTCCTCGATTTGGTCG | XM_008772052.2 |
| FLG (Rat) | CTGACACCTCCAGTCGCTC | GTCTGTCGACTGCCCATGAC | XM_008761264.2 |
Q-RT-PCR: quantitative real-time PCR.