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. 2023 Nov 17;103(2):103293. doi: 10.1016/j.psj.2023.103293

Figure 2.

Figure 2

ucOCN activates the ADPN-AMPK/PPARα-mTOR signaling pathway in the treated chicken embryonic hepatocytes. (A) Immunoblot of p-mTOR, mTOR, p-AMPK, AMPK, PPARα and ADPN protein; (B) The changes of p-mTOR/mTOR ratio; (C) The changes of p-AMPK/AMPK ratio; (D) The changes of PPARα protein level; (E) The changes of ADPN protein level; (F) The changes of ADPN mRNA expressions; (G) The changes of AdipoR1 mRNA expressions; (H) The changes of AdipoR2 mRNA expressions; (I) The changes of PPARα mRNA expressions. The data represent mean ± SEM (n = 6 per group). Differences were determined by 1-way ANOVA followed by LSD test. *P < 0.05 compared with the control group, #P < 0.05 compared with the FE group. Abbreviations: AdipoR1, adiponectin receptor 1; AdipoR2, adiponectin receptor 2; ADPN, adiponectin; AMPK, adenosine 5′-monophosphate (AMP)-activated protein kinase; FE, fat emulsion; mTOR, the mammalian target of rapamycin; PPARα, peroxisome proliferators-activated receptor α; ucOCN, undercarboxylated osteocalcin.