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. 2023 Nov 17;103(2):103293. doi: 10.1016/j.psj.2023.103293

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© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

AdipoRon activates lipophagy in the FE-induced chicken embryonic hepatocytes. (A) Immunoblot of the autophagy-related protein level of p62, Beclin-1, LC3-Ⅰ, and LC3-Ⅱ; (B) The change of p62 protein expression; (C) The change of Beclin-1 protein level; (D) The change of LC3-Ⅱ/LC3-Ⅰ ratio; (E) The colocalization of LC3 and LDs in FE-induced chick embryo hepatocytes. Red: Immunofluorescence of LC3 puncta, Green (BODIPY): Immunofluorescence of LDs, Blue: Immunofluorescence of DAPI. Merge showed the colocalization of LC3 and LDs. The data represent mean ± SEM (n = 6 per group). Differences were determined by 1-way ANOVA followed by LSD test. *P < 0.05, compared with the control group, #P < 0.05 compared with the FE group. Abbreviations: Beclin-1, yeast ATG6 homologue; FE, fat emulsion; LC3-Ⅰ, microtubule-associated proteins light chain 3-Ⅰ; LC3-Ⅱ, microtubule-associated proteins light chain 3-Ⅱ; P62, P62/SQSTM1; ucOCN, undercarboxylated osteocalcin.