FIG. 3.

Schematic representations of the genomic strands of BUN-S(ren), AG-CB, BUN-S(ren)-C/G, and AG-CB-U/G templates and analysis of their RNA synthesis activities. (A) Templates AG-CB-U/G and BUN-S(ren)-C/G were derived from template AG-CB, and their nucleotide differences from the genomic strand of wild-type template BUN-S(ren) are shaded. The potential to form Watson-Crick base-pairs (*) or noncanonical U-G pairings (•) are also shown. (B) The RNA synthesis activities of BUN-S(ren), AG-CB, AG-CB-U/G, and BUN-S(ren)-C/G were analyzed by direct visualization of metabolically labeled actinomycin D-resistant RNAs using agarose-urea gel electrophoresis. BUNV-specific RNAs are marked with arrowheads.