FIG. 4.

Analysis of the RNA synthesis-signaling abilities of 3′ and 5′ nucleotide position 9 of the genomic template. (A) The nucleotide identity of 3′ and 5′ genomic position 9 was changed from the wild-type combination (U-G) to all other possible permutations. (B) The RNA synthesis characteristics of these altered templates were analyzed by direct visualization of metabolically labeled actinomycin D-resistant RNAs using agarose-urea gel electrophoresis. BUNV-specific RNAs are marked with arrowheads.