FIG. 5.

Primer extension analysis of the RNA synthesis-signaling abilities of BUNV templates having 3′ and 5′ position 9 combinations of U-A, C-A, G-A, and A-A to detect transcription from the antigenomic strand. RNAs were harvested from vTF7-3-infected BHK-21 cells transfected with a cDNA expressing a genome analog and either BUNV S and L support plasmids (pL) (+) or BUNV S support plasmid alone (−). (A) Positive-sense RNAs generated from genomic strands were detected using the negative-sense oligonucleotide BUNMRNA. The variously sized mRNA 5′ extensions are marked with a vertical bar. (B) Negative-sense RNAs generated from the antigenomic strand were detected using oligonucleotide minus-80. In each panel, the cDNA expressing template BUN-S(ren) was sequenced with the corresponding end-labeled oligonucleotide to act as a size marker.