Fig. 3.

In vitro shuttling of Ola1p between mitochondria and LDs. A: Mitochondria containing Ola1p-GFP and nonlabeled LDs were isolated. Both organelles were mixed in a reaction tube and after 30 min of incubation separated again. The fluorescence of both organelles was either measured in a fluorimeter plate reader or detected by Western blot. B: The fluorescence of both organelles after combination and separation is shown. The dashed line represents mitochondria, and the continuous line represents LDs. The x-axis represents increasing amounts of LDs (e.g., 9× is a 9-fold excess of LDs).