FIG. 1.

RNase protection assay of transcripts from transfected reporter template. A 1,706-bp fragment of the HPV31 genome extending from the 3′ end of the L1 gene through the upstream regulatory region (URR) and E6 and E7 genes to the 5′ end of the E1 gene was cloned into the pGL2B luciferase reporter vector to create pGL2B-1706 (fragment A). This plasmid or the empty vector was transfected into HFK cells and incubated in monolayer culture. After 48 h, mRNA was isolated and RNase protection analysis was performed with a riboprobe specific for transcripts in the p742 region. The RNA analyzed in each lane is as follows: A, mRNA from HFKs transected with the fragment A reporter; pGL2B, mRNA from HFKs transfected with the empty vector; HPV31a, total RNA from monolayer HFK31a:1 cells; Y+ and Y−, total yeast RNA with and without nuclease digestion, respectively. A darker exposure of the HPV31a lane (*) from the same gel is shown to the right.