TABLE 1.
Biological properties of WT CCMV and BMV and their variants used in this study
| Inoculuma | Symptomsb
|
|||
|---|---|---|---|---|
|
C. quinoa
|
Cowpea
|
|||
| L | S | L | S | |
| CCMV (WT) | NL | NI | RLL | SM |
| C3/Δ919 | NL | NI | RLL | NI |
| C3/P10 | NT | NT | RLL | SM |
| C3/P13 | NT | NT | RLL | SM |
| C3/P18 | NT | NT | RLL | SM |
| C3/P19 | NT | NT | RLL | SM |
| BMV (WT) | CLL | SM | NI | NI |
| B3/CCPΔ919 | NL | NI | NI | NI |
| C3/BCPΔ919 | NLc | NI | RLLc | NI |
a
Each inoculum contained a mixture of WT RNA1 and RNA2 transcripts and desired WT or mutant RNA3 transcript adjusted to a concentration of 150 μg/ml.
b
L, local; S, systemic; NL, necrotic local lesions; CLL, chlorotic local lesions; RLL, reddish necrotic local lesions; SM, systemic mottling; NI, not infected, NT, not tested.
c
In each case, few local lesions that are hard to visualize appeared 3 to 4 weeks post-inoculation.