TABLE 1.
Genes that were significantly altered by siRNAsa
| Cell line or gene no. | Accession no. | Protein encoded by gene |
|---|---|---|
| HeLa cells | ||
| 25 | N75719 | Serine (or cysteine) proteinase inhibitor E1 (SERPINE1) |
| 26 | AI989728 | Serine (or cysteine) proteinase inhibitor B5 (mapsin) (SERPINB5) |
| 28 | AI540460 | Prostaglandin E synthase (PTGES) |
| 39 | W65461 | Dual specificity phosphatase 5 (DUSP5) |
| 44 | H72027 | Getsolln (Finnish type) (GSN) |
| 48 | AI982657 | Growth differentiation factor 15 (MIC-1) (GDF15) |
| 51 | AA480815 | Immediate early response 3 (IER3) |
| 53 | H84481 | EphA2 (EPHA2) |
| 55 | AA453410 | Tumor necrosis factor receptor superfamily 10B (TNFRSF10B) |
| 1 | AA485865 | Interleukin 7 receptor (IL7R) |
| 2 | N30553 | Pregnancy-specific beta-1-glycoprotein 4 (PSG4) |
| 3 | T95747 | Pregnancy-specific beta-1-glycoprotein 1 (PSG1) |
| 4 | R02384 | Pregnancy-specific beta-1-glycoprotein 6 (PSG6) |
| 5 | H12630 | Pregnancy-specific beta-1-glycoprotein 3 (PSG3) |
| 6 | R73672 | Clone IMAGE:3881549 |
| 7 | AI659145 | Serum amyloid A2 (SAA2) |
| 8 | AA411619 | Cathepsin S (CTSS) |
| 9 | AI394388 | EST |
| 10 | AA019270 | Formin (limb deformity) (clone IMAGE:4213061) |
| 11 | AA479609 | GPI-anchored metastasis-associated protein homolog (C4.4A) |
| 12 | AA977631 | TNF receptor-associated factor 1 (TRAF1) |
| 13 | AA425806 | Deformed epidermal autoregulatory factor 1 (DEAF1) |
| 14 | AA436152 | Semaphorin 5A (SEMA5A) |
| 15 | R07870 | Baculoviral IAP repeat-containing 3 (BIRC3) |
| 16 | W68159 | Platelet-derived growth factor beta polypeptide (PDGFB) |
| 17 | R20886 | Stanniocalcin 2 (STC2) |
| 18 | W46900 | Chemokine (C-X-C motif) ligand 1 (CXCL1) |
| 19 | AI200635 | EST |
| 27 | H75832 | Transmembrane protein 2 (TMEM2) |
| 29 | T99236 | Jun B proto-oncogene (JUNB) |
| 33 | AI148233 | Interleukin 11 (IL11) |
| 34 | R44077 | Ectonucleoside triphasphate diphosphohydrolase (ENTPOT) |
| 35 | AI085559 | KIAA1363 protein (KIAA1363) |
| 36 | AA458965 | Natural killer cell transcript 4 (NK4) |
| 37 | AA644211 | Prostaglandin-endoperoxide synthase 2 (PTGS2) |
| 38 | R38539 | Fibroblast growth factor 2 (FGF2) |
| 40 | AA952897 | Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (NFKB2) |
| 41 | AA293653 | Phosphoprotein enriched in astrocytes 15 (PEA15) |
| 42 | R70685 | Jagged 1 (Alagille syndrome) (JAG1) |
| 43 | AA029934 | Integrin, alpha V (antigen CD51) (TGAV) |
| 45 | AI418596 | Netrin 4 (NTN4) |
| 48 | AJ214697 | Follistatir (FST) |
| 47 | AA046311 | FLJ42179 |
| 49 | N98591 | Interleukin 6 (interferon, beta 2 (IL6) |
| 50 | AA011335 | Melanoma antigen recognized by T cells 2 (MART2) |
| 54 | AI000677 | Sialyltransferase 4A (SIAT4A) |
| 57 | AA463610 | Integrin, alpha 2 (CD49B) (ITGA2) |
| 59 | AA488609 | Nucleoporin 88 kDa (NUP88) |
| 61 | AA432103 | LIM protein (similar to rat protein kinase C-binding enigma) (LIM) |
| 62 | T60191 | Monoglyceride lipase (MGLL) |
| 63 | AW007267 | Laminin, beta 3 (LAMB3) |
| 64 | AI435445 | CD109 antigen (CD109) |
| 65 | AA017170 | Iduronate 2-sulfatase (Hunter syndrome) (IDS) |
| 66 | H52739 | PDZ domain containing 3 (PDZK3) |
| 67 | AA877213 | Cytochrome P450 24A1 (CYP24A1) |
| 91 | AI963014 | Tumor necrosis factor, alpha-induced protein 3 (TNFAIP3) |
| Caski cells | ||
| 75 | AA405449 | Damage-specific DNA binding protein 2, 48kDa (DDB2) |
| 83 | AA903201 | Notch 1 (NOTCH1) |
| 20 | AA504624 | Inositol polyphosphate-5-phosphatase (INPP50) |
| 21 | W84711 | Fibronectin 1 (FN1) |
| 22 | H09620 | FLJ31869 |
| 23 | AI678132 | Hairy and enhancer of split homolog 2 (HES2) |
| 24 | AI123041 | Serine (or cysteine) proteinase inhibitor B7 (megsin) (SERPINB7) |
| 78 | AA669057 | Pyroglutamyl-peptidase I (PGPEP1) |
| 88 | AA496957 | FLJ21245 |
| 87 | H90930 | N-Acylsphingosine amidohydrotase 3-like (ASAH3L) |
| 88 | AA497002 | Metanoma cell adhesion molecule (MCAM) |
| 89 | AA521292 | ATP-binding cassette A1 (ABCA1) |
| 90 | AA001432 | Laminin, alpha 3 (LAMA3) |
| 94 | AA453754 | Serine/threonine kinase 17a (STK17A) |
| 95 | AI091648 | Cathepain B (CTSB) |
| 96 | N50998 | Synapsin II (SYN2) |
| 97 | AA482287 | Protein tyrosine phosphatase IVA1 (PTP4A1) |
| 98 | AA132964 | Interleukin 22 receptor, alpha 1 (L22RA1) |
| 99 | AW075585 | Lenascin C (hexabrachion) (TNC) |
| SiHa cells | ||
| 30 | AA126115 | FXYD domain containing ion transport regulator 3 (FXYD3) |
| 52 | H71883 | Tumor necrosis factor receptor 10d (TRUNDD) (TNFRSF10D) |
| 31 | AI924385 | Lymphocyte antigen 6D (LY6D) |
| 32 | N25945 | Phosphoipid scramblase 1 (PLSCR1) |
| 74 | AA975612 | Pleckstrin homology-like domain A3 (PHLDA3) |
| HeLa and Caski cells | ||
| 76 | AI671604 | Fodoxin reductase (FDXR) |
| 65 | AI122802 | Ribonucleotide reductase M2 B (RRM2B) |
| 92 | H12043 | Cytoplasmic FMR1 interacting protein 2 (CYFIP2) |
| 56 | AA973861 | FLJ00310 |
| 60 | AA258396 | Pleckstrin homology-like domain A1 (PHLDA1) |
| 82 | AA156054 | Ribosomal protein S27-like (RPS27L) |
| 93 | N99256 | Chromosome 7 open reading frame 10 (C7orf10) |
| HeLa, SiHa, and Caski cells | ||
| 68 | AA447651 | Sestrin 1 (SESN1) |
| 69 | H69582 | BTG 2 |
| 70 | AA459364 | Tumor protein p53 inducible nuclear protein 1 (TP53INP1) |
| 71 | AI688112 | BCL2 binding component 3 (BBC3) |
| 73 | AI952615 | Cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A) |
| 77 | AA083407 | Tripartite motif-containing 22 (TRIM22) |
| 79 | AA214617 | Hdm2, transformed 3T3 cell double minute 2 (HDM2) |
| 58 | AA682514 | FLJ12484 |
| 72 | R76883 | Growth arrest-specific 6 (GAS6) |
| 80 | N50744 | MGC5370 |
| 81 | H79861 | FLJ20291 |
| 84 | W56582 | FLJ31683 |
a
Gene numbers correspond to the genes indicated in Figure 3. Genes are grouped according to the cell line(s) in which they were significantly altered by siRNAs against E6 or E6AP. Genes shown in bold are those that are known to be induced, either directly or indirectly, by p53.