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. 2023 Aug 11;6(6):573–584. doi: 10.1002/ame2.12342

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© 2023 The Authors. Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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PF4 attenuated the osteogenic differentiation ability of BMMSCs in vitro. (A) Representative image of ALP and ARS staining of BMMSCs in each of the five groups. Scale bar = 100 μm. (B) ALP quantitative analysis and (C) ARS semiquantitative analysis of BMMSCs were evaluated in each of the five groups. N = 6 samples per group. Data are expressed as the mean ± standard deviation (SD) of experiments. ALP, alkaline phosphatase; ARS, Alizarin Red S; BMMSCs, bone marrow mesenchymal stem cells; OM, osteogenic medium; PF4, platelet factor 4.