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. 2023 Aug 11;6(6):573–584. doi: 10.1002/ame2.12342

FIGURE 7.

FIGURE 7

PF4 decreased the ALP (alkaline phosphatase) activity of BMMSCs by inhibiting the ITGA5‐FAK‐ERK pathway. (A) The protein expression levels of ITGA5, p‐FAK, FAK, p‐ERK1/2, and ERK1/2 were evaluated among the CONT‐LV, OM, ITGA5i‐LV, and ITGA5‐LV groups. (B) Representative images of staining and quantitative analysis of ALP for BMMSCs among the CONT‐LV, OM, ITGA5i‐LV, and ITGA5‐LV groups. Scale bar = 100 μm. N = 3–6 samples per group. Data are expressed as the mean ± standard deviation (SD) of the experiments. BMMSCs, bone marrow mesenchymal stem cells; CONT‐LV, control lentivirus; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; ITGA5, integrin α5; ITGA5‐LV, ITGA5 overexpression lentivirus; ITGA5i‐LV, ITGA5 interfering lentivirus; OM, osteogenic medium; PF4, platelet factor 4; p‐ERK, phosphorylated extracellular regulated protein kinases; p‐FAK, phosphorylated focal adhesion kinase.