FIG. 3.

TLR3 RNAi impairs CCL5 and CXCL10 production but has no effect on CXCL8 synthesis or viral replication in MRC-5 cells. TLR3 siRNA and random siRNA controls were transfected into MRC-5 cells and infected with RSV (MOIs of 0.5 to 1). Expression levels of TLR3 (A), RSV mRNA (B), and chemokines (D to F) were determined at 24 and 48 hpi. MRC-5 cells transfected with TLR3 siRNA versus random siRNA controls differed significantly in TLR3 expression and production of CCL5 at 48 hpi (*, P < 0.05). At 48 hpi, a plaque assay was performed to determine the viral load in cells with altered TLR3 expression (C). Data are representative of three experiments, with n = 3 per treatment per experiment. Each time point represents the mean ± standard error of the mean. TLR3i, TLR3 siRNA.