FIG. 4.

TLR3 RNAi impairs CCL5 and CXCL10 production but has no effect on CXCL8 synthesis or viral replication in A549 cells. TLR3 siRNA and random siRNA controls were transfected into MRC-5 cells and infected with RSV (MOI of 1). Levels of TLR3 expression (A), viral replication (B), and chemokines (C to E) were determined at 24 and 48 hpi. A549 cells transfected with TLR3 siRNA versus random siRNA controls differed significantly in TLR3 expression and synthesis of CCL5 and CXCL10 at 48 hpi (*, P < 0.05). Cells were harvested at 48 hpi postinfection, and a plaque assay was performed (B). Data are representative of three experiments, with n = 3 per treatment per experiment. Each time point represents the mean ± standard error of the mean. TLR3i, TLR3 siRNA.