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IFN-α/β signaling is not required for RSV-induced chemokine production. A549 cells were pretreated for 1 h before RSV infection with anti-IFN-α/β receptor (aIFNR) antibody (10 μg/ml), immunoglobulin G (IgG; 10 to 20 μg/ml), or medium. The antibody was left in the medium and cells were infected at an MOI of 0.5. At 48 hpi, RNA levels of CXCL8 and CCL5 were analyzed.
