FIG. 5.

Virus titers in brain tissue of infected mice, sacrificed at 5 days postinfection. Viral titers of clarified brain homogenates (five mice/virus) were determined by plaque assay in DBT cells. The titer for each sample was determined in two separate plaque assays, with each point the average of two measurements and normalized for brain mass. Means for five mice are indicated. Titers for VUSS0 and VUSS2 were reproducible in separate plaque assays, with plaques detected only in undiluted specimens.