TABLE 3.
Amino acid sequences of virus plaque isolates from mouse brains infected with RA59, VUSS3, VUSS0, and VUSS2a
| Strain and mouse no. | Plaque isolate(s) | ORF1b p59-nsp14 aa6398 | ORF2a 30-kDa aa94 |
|---|---|---|---|
| RA59 | TYR | LEU | |
| Mouse 3 | 1 | Tyr | Leu |
| Mouse 4 | 1 | Tyr | Leu |
| VUSS3 | TYR | LEU | |
| Mouse 2 | 1 | Tyr | Leu |
| Mouse 3 | 1 | Tyr | Leu |
| VUSS0 | HIS | PRO | |
| Mouse 1 | 1, 3, 4 | (Tyr) | (Leu) |
| Mouse 1 | 5 | (Tyr) | Pro |
| Mouse 1 | 6 | His | Pro |
| Mouse 2 | 1 | His | Pro |
| Mouse 3 | 1, 2 | His | Pro |
| Mouse 4 | 1, 2 | His | Pro |
| Mouse 5 | 1, 2 | His | Pro |
| VUSS2 | HIS | LEU | |
| Mouse 1 | 1, 2 | His | Leu |
| Mouse 2 | 1, 2 | His | Leu |
| Mouse 4 | 1, 3, 4, 5, 6 | (Tyr) | (Pro) |
| Mouse 5 | 1 | His | Leu |
a
Input sequence is indicated by bold capitals. Changes from input sequence are indicated by parentheses. VUSS0 mouse 5 isolates both contained an A to C mutation coding Asp to Ala change at aa 53 in ORF2a 30-kDa protein.