FIG. 1.

IVK of HIV-1 Gag-pulsed cells. Equal numbers of autologous PBMC pulsed either with a pool of overlapping HIV-1 Gag peptides (SNARF, red) or without Gag peptides (CFSE, green; control population) were infused intravenously. Circulating labeled cell numbers in the peripheral blood were identified by flow cytometry at various time points thereafter and are expressed as values relative to those of the control population. (A) Similar numbers of circulating Gag-pulsed and control cells are identified 5 min following infusion but selective depletion of the Gag-pulsed population occurs 20 h following infusion in an HIV-1 immune macaque. (B) Lymph node mononuclear cells examined for the loss of Gag-pulsed cells 36 h following infusion in an unimmunized macaque and a preimmune macaque are shown.