FIG. 2.

In vivo SHIV-specific killing after immunization and pathogenic SHIV infection. Flow cytometric analysis of macaque peripheral blood following infusion of PBMC pulsed with sets of overlapping SIV peptides: Pol (SNARF, red); Gag (CFSE^lo, green), and Nef or DMSO (CFSE^hi, green; control population). Cell numbers are expressed as values relative to those of the control population. (A) Progressive elimination of Gag-pulsed cells in a DNA- and FPV-immunized animal was detected 4 and 16 h following infusion; no Gag or Pol-specific cytotoxicity was detected in an unimmunized naïve animal. (B) At 14 days after SHIV challenge, near-complete elimination of Gag- and Pol-pulsed cells in a previously immunized animal occurred.