FIG. 5.

Induction of CD4⁺- and CD8⁺-T-cell immunity. SHIV-specific T-cell responses measured by IFN-γ ICS after infusion of SIV Gag- and Pol-peptide-pulsed PBMC and whole blood is shown. (A) Flow cytometric analysis of increased CD8⁺ and CD8⁻ (CD4⁺) SIV Gag-specific T cells before (day 0) and after (day 17) infusion of peptide-pulsed PBMC in a previously immunized and SHIV-infected macaque. Percents CD3⁺ CD8⁺ and CD3⁺ CD4⁺ SIV Gag-specific lymphocytes are indicated in upper right and left quadrants, respectively. (B) Kinetic analysis of CD4⁺- and CD8⁺-T-cell responses to SIV Gag and Pol following infusion of Gag- and Pol-peptide-pulsed PBMC into two macaques previously immunized and challenged with SHIV_mn229 (H8 [▪] and H20 [♦]). (C) Analysis of T-cell responses following infusion of Gag- and Pol-peptide-pulsed whole blood in three macaques previously infected with SHIV_sf162P3 (9E [▪], 23E [♦], and 24E [•]).