Skip to main content
. 2005 Mar;79(6):3479–3487. doi: 10.1128/JVI.79.6.3479-3487.2005

FIG. 1.

FIG. 1.

Construction of a recombinant HHV8 BAC with a large deletion in ORF50. (A) Schematic showing the region deleted from within ORF50 and the resultant configuration of the genome after the deletion. Also depicted are the HindIII sites used to confirm the proper BAC construction and the position of the hybridization probe. (B) Southern blot of HindIII-cleaved BAC36 and BAC36Δ50 DNAs hybridized with a probe specific for the ORF50 region. Lanes: 1, BAC36Δ50; 2, BAC36. Also shown is an ethidium bromide-stained gel of BAC36Δ50 and BAC36 DNA cleaved with HindIII. Lanes: 3, molecular size markers; 4, BAC36Δ50 DNA; 5, BAC36 DNA.