FIG. 2.

Complementation of BAC36Δ50 virus production by expression of K-Rta. For complementation, BAC-containing 293 cell lines were treated with TPA or 293-LAC50 cells were transfected with BAC36Δ50 and induced to express K-Rta by treatment with IPTG. Supernatants from each treatment were harvested 4 days postinduction and used to inoculate fresh 293 cells. (A) Cells were inoculated with supernatant from BAC36-containing 293 cells treated with TPA. (B) Cells were inoculated with supernatant from BAC36Δ50-containing 293 cells treated with TPA. (C) Cells were inoculated with supernatant from BAC36Δ50-transfected 293-Lac50 cells treated with IPTG. (D) IPTG induction of ORF50 complements the defect in BAC36Δ50. 293-LAC50 cells were transfected with BAC36 or BAC36Δ50 and treated with IPTG. Real-time PCR was performed, and the mRNA accumulation for K8 was determined.