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BAC36Δ50 failed to express ORF59 and K8.1 proteins. 293 cells containing BAC36 (wild-type BAC) or BAC36Δ50 were treated with TPA, fixed, and reacted with monoclonal antibodies specific for LANA, ORF59, or K8.1 viral proteins. Cells containing BAC constructs are positive for green fluorescent protein. Cells positively reacting with antibodies are shown as red-fluorescing cells.
