FIG. 1.
Construction of virus mutants. (A) A schematic map of the PrV genome showing the unique long (UL) and unique short (US) regions, the inverted repeat sequences (IR, TR), and the positions of the BamHI restriction fragments which are numbered according to their size. (B) Enlargement of the UL51 gene region. The UL51 gene is transcribed into a unique mRNA antiparallel to the neighboring genes UL50, coding for dUTPase, and UL52, encoding the putative primase subunit of the primase/helicase complex (6). A region of reiterated sequences located between ORF-1 and UL54 is shown by a hatched box. Relevant restriction sites are indicated. (C) Construction of PrV-ΔUL51F. Deletion of UL51-specific sequences between the StuI sites is indicated. Locations of the polyadenylation signals are shown by hollow arrows (pointing downward for the UL50 gene and pointing upward for the UL51 gene). (D) Amino-terminal (UL51N) and carboxy-terminal (UL51C) regions used for the generation of specific antisera.