FIG. 1.
Coimmunoprecipitation reveals complex formation among M139, M140, and M141 gene products. The figure shows autoradiographs of radiolabeled proteins immunoprecipitated from lysates of NIH 3T3 cells either mock infected, infected with WT MCMV, or infected with mutant MCMV having gene M139 (Δ139), M140 (Δ140), or M141 (Δ141) deleted. Cells were infected using 1 PFU/cell, radiolabeled from 20 to 24 h postinfection, and harvested in immunoprecipitation lysis buffer. The rabbit polyclonal antibodies used for each immunoprecipitation are denoted above each lane. “Preimm” signifies use of preimmune rabbit serum.