TABLE 2.

Stability of CD4 T-cell response between weeks 140 and 191 (2.7 and 3.7 years) postchallenge

Animal group and no.a	No. of CD4 epitopes	Peptide	Sequenceb	% CD4c
				IFN-γ		IFN-γ + IL-2
				2003	2004	2003	2004
2.5 mg i.d.
*1	1	Gag 46	CTPYDINQMLNCVGD	0.01	0.02	0.01	0.01
*2	1	Gag 46	CTPYDINQMLNCVGD	0.04	0.06	0.01	0.02
*3	1	Gag 46	CTPYDINQMLNCVGD	0.02	<	<	<
4	1	Env 43	KVKKEYALFNRLDVV	0.03	0.07	0.02	0.02
5	4	Gag 21	TVCVIWCIHAEEKVK	0.04	0.08	0.01	0.01
		Gag 40	WVKLIEEKKFGAEVV	0.23	0.17	0.07	0.01
		Gag 62	EQIQWMYRQQNPIPV	0.09	<	0.03	<
		Env 11	VPVWREATTTLFCAS	0.04	0.07	0.01	0.03
6	2	Gag 39	TLNAWVKLIEEKKFG	0.09	0.29	0.05	0.13
		Gag 62	EQIQWMYRQQNPIPV	0.07	0.31	0.04	0.19
0.25 mg i.d.
*13	1	Gag 46	CTPYDINQMLNCVGD	0.02	<	0.02	<
*14	3	Gag 21	TVCVIWCIHAEEKVK	0.02	0.03	0.01	0.02
		Gag 46	CTPYDINQMLNCVGD	0.04	0.05	0.03	0.04
		Env 11	VPVWREATTTLFCAS	0.02	0.02	0.01	0.01
15	0
17	2	Gag 26	KQIVQRHLVVETGTT	0.02	<	<	<
		Gag 49	VGDHQAAMQIIRDII	0.03	<	0.01	<
18	3	Gag 38	LSPRTLNAWVKLIEE	0.02	0.02	0.01	0.01
		Gag 49	VGDHQAAMQIIRDII	0.03	0.05	0.02	0.02
		Gag 65	IPVGNIYRRWIQLGL	0.02	0.01	0.01	0.01
2.5 mg i.m.
*7	0
*8	3	Gag 95	IPFAAAQQRGPRKPI	0.01	0.01	0.01	0.01
		Env 142	LTVWGIKQLQARVLA	0.02	<	<	<
		Env 167	SLWNWFDITNWLWYI	0.03	<	0.01	<
*10	1	Gag 46	CTPYDINQMLNCVGD	0.03	0.06	<	<
12	1	Gag 46	CTPYDINQMLNCVGD	0.04	0.03	0.02	0.01
0.25 mg i.m.
*20	1	Gag 46	CTPYDINQMLNCVGD	0.02	0.08	0.01	0.01
*21	0
23	5	Gag 50	QAAMQIIRDIINEEA	0.11	0.11	0.05	0.04
		Gag 60	AGTTSSVDEQIQWMY	0.03	0.02	<	0.01
		Gag 66	NIYRRWIQLGLQKCV	0.05	0.06	0.02	0.02
		Gag 79	AVKNWMTQTLLIQNA	0.04	0.02	0.01	0.01
		Gag 105	CGKMDHVMAKCPDRQ	0.09	0.08	0.04	0.03
24	0
Unvaccinated control
30	2	Gag 25	TEEAKQIVQRHLVVE	0.03	0.15	<	0.05
		Gag 46	CTPYDINQMLNCVGD	0.04	0.11	<	0.01

^aMeasurements for the surviving control are at 2.4 and 3.4 years. *, animals with the A*01 histocompatibility type.

^bConsecutive positive peptides with 11 overlapping amino acids were considered to be a single epitope and are given as one peptide with the overlapping amino acids in bold. CTPYDINQM, Gag-CM9 dominant A*01 epitope.

^cResponses that showed a >2-fold increase are in bold, and responses that showed a >2-fold decrease are italic. <, below detection.