FIG. 1.

(A) Physical and genetic map of plasmid pNZ4000. The eps gene cluster is located between IS982 and orfY. (B) Physical and genetic maps of the replication and mobilization regions of pNZ4000. The fragments used for functional analysis are depicted below. A, AccI; C, ClaI; E, EcoRI; H, HincII; N, NcoI; O, XhoI; P, SphI; S, Sau3AI; V, NspV; X, XbaI. For AccI, ClaI, HincII, NcoI, NspV, and Sau3AI, only sites relevant for subcloning are included. Sequences are available under GenBank accession no. AF036485, AF03686, and AF03687.