Skip to main content
. 2005 Mar;79(6):3883–3887. doi: 10.1128/JVI.79.6.3883-3887.2005

FIG. 2.

FIG. 2.

Detection of DNA sequences of a new polyomavirus in clinical sample 12 derived from a chimpanzee. (A) PCR products of seven field samples (#11 to #17), SV40-infected Vero cells (SV-40), and the negative control [(−) Ctrl] were separated on ethidium bromide-stained 2% agarose gels. (B) Analysis of products amplified with primers VP1-1f and VP1-1r (1st PCR) and primers VP1-2f and VP1-2r (2nd PCR) with single PCR protocols or with the first PCR followed by the second PCR in a nested PCR protocol by ethidium bromide-stained 2% agarose gel electrophoresis. The template DNA was derived from sample 12, SV40-infected Vero cells (SV-40), or the negative control [(−)-Ctrl]. M, DNA ladder mix (Fermentas). (C) Phylogenetic relationships of the new polyomavirus (ChPyV) with 10 other polyomaviruses, based on the nucleotide sequences of the whole VP1-encoding region, aligned by the ClustalW method.