TABLE 1.

Oligonucleotides used in polyomavirus broad-spectrum PCRs

PCR	Primer designation	Sequencea (5′-3′)	Product length (bp)
VP1 specific
1st PCR	VP1-lf	CCAGACCCAACTARRAATGARAA	829-1137
	VP1-1r	AACAAGAGACACAAATNTTTCCNCC
Nested PCR	VP1-2f	ATGAAAATGGGGTTGGCCCNCTNTGYAARG	249-273
	VP1-2r	CCCTCATAAACCCGAACYTCYTCHACYTG
VP3-VP1 specific
1st PCR	VP3-1f	CTCCAGGAGGTGCAMABCAAMG	562-631
	VP3-1r	TGCAATTCCAGAGGTTCNCCNCCNAC
Nested PCR	VP3-2f	ACTGGATGCTGCCTYTAMTTYTAGG	394-459
	VP3-2r	TCAGTTTTTACAGTTACWGCYTCCCACAT
T-Ag specific
1st PCR	T-1f	GATGTTTCCTTTCTARRTTNAC	536-551
	T-1r	GCAAAGATCAAAAAAGCATHTGYCA
Nested PCR	T-2f	AAATGATCTCTCAAGTTATCNARRTT	208-235
	T-2r	AAAGGTCCAGTTAATAGTGGNAARAC

^aB,C+G+T; H,A+C+T; M,A+C; N,A+C+G+T; R,A+G; W,A+T; Y,C+T.