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. Author manuscript; available in PMC: 2023 Dec 30.
Published in final edited form as: Genesis. 2023 Jan 9;61(1-2):e23509. doi: 10.1002/dvg.23509

FIGURE 4.

FIGURE 4

Inhibition of p53 during late embryonic stage partially rescued the premature fusion of ISS in P0-Cre;caBmpr1a mice. Pifithrin-α (PFT), an inhibitor for p53, was injected to the pregnant mice from embryonic day 15.5 (E15.5) to E18.5, then the skulls of control and P0-Cre;caBmpr1a mice were stained with alizarin red and alcian blue (skeletal preparation) following the pups were harvested at postnatal day 17. Two phenotypes of P0-Cre;caBmpr1a mice were found by PFT injection; phenotype A (4 out of 7 pups) and phenotype B (3 out of 7 pups). Skeletal preparation of the cranial base (a–c) and calvaria (d–f) are shown. (g) Schematic measurement points of angular and length are shown. (h–o) Measurements with significant differences between control (white bar), P0-Cre;caBmpr1a phenotype A (gray bar) and P0-Cre;caBmpr1a phenotype B (red bar) were shown. Scale bar; 2 mm. One-way ANOVA with Turkey's test was used for statistical analyses. **p < .01, *p < .05.