TABLE 2.

Mutations in nicking substrates and 18-mers

Designationa	Sequenceb	Mobilityc change	Nicking (% wild type)d
S1-WTe	GGCGGCGGT TGGGGCTCG	0	100
S1-G2A-C3T-G4A	GataGCGGT TGGGGCTCG	Slower	18*
S1-G2A-G5A	GaCGaCGGT TGGGGCTCG	0	NT
S1-G1A	aGCGGCGGT TGGGGCTCG	0	102
S1-G2A	GaCGGCGGT TGGGGCTCG	0	66*
S1-C3T	GGtGGCGGT TGGGGCTCG	Slower	44*
S1-G4A	GGCaGCGGT TGGGGCTCG	0	109
S1-G5A	GGCGaCGGT TGGGGCTCG	0	99
S1-C6T	GGCGGtGGT TGGGGCTCG	Slower	92
S1-G7A	GGCGGCaGT TGGGGCTCG	0	70
S1-G8A	GGCGGCGaT TGGGGCTCG	0	90*
S1-T9C	GGCGGCGGc TGGGGCTCG	0	12*
S1-T10C	GGCGGCGGT cGGGGCTCG	Faster	21*
S1-G11A	GGCGGCGGT TaGGGCTCG	0	81
S1-G12A	GGCGGCGGT TGaGGCTCG	0	110
S1-G13A	GGCGGCGGT TGGaGCTCG	0	102
S1-G14A	GGCGGCGGT TGGGaCTCG	Slower	30*
S1-C15T	GGCGGCGGT TGGGGtTCG	Slower	23*
S1-T16C	GGCGGCGGT TGGGGCcCG	Faster	66*
S1-C17T	GGCGGCGGT TGGGGCTtG	0	108
S1-G18A	GGCGGCGGT TGGGGCTCa	0	22*

^aUnderlined bases are conserved between AAVS1 and AAV-2-ITR.

^bMutated bases are in lowercase. For nicking substrates, complementary mutations were made in the other strand.

^cThe mutant 18-mer oligonucleotide shown migrates faster than, slower than, or about the same as (0) wild-type S1-18 as indicated.

^dNT, not tested. *, Significantly less than or greater than S1-WT (P < 0.05).

^eWild-type sequence.