Figure 2.

Hypoxic preconditioning increases cell survival in vitro. (A): Schematic overview of experimental design: mesenchymal stem cells/multipotent stromal cells (MSCs) were cultured in complete culture media (supplemented with 10% FBS) for varying times in hypoxia (1% O₂). Then (time points A and B), culture media were changed to MEMa without supplements (0% FBS), with no further media changes, and incubated in 1% O₂ for up to 12 days. (B): After preincubation of MSCs for 0, 16, 48, or 96 hours in hypoxia, cells were changed to the nutrient and oxygen limited environment. Cells were counted using a hemocytometer and Trypan blue exclusion dye every 3 days. Cell survival is expressed as a percentage of remaining cells relative to day 0, after HP (n = 6). Statistical analysis was performed by ANOVA; *, p < .05. Abbreviations: FBS, fetal bovine serum; HP, hypoxic preconditioning.